ARG63205
anti-GRB7 antibody
anti-GRB7 antibody for Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GRB7 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | This antibody recognises the longer isoform of this protein in A431 lysate. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GRB7 |
| 抗原物种 | Human |
| 抗原 | ELDLSPPHLSSSPE-C |
| 偶联标记 | Un-conjugated |
| 別名 | Epidermal growth factor receptor GRB-7; Growth factor receptor-bound protein 7; B47; GRB7 adapter protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14451 Human Growth factor receptor-bound protein 7 |
|---|---|
| 背景介绍 | The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 60 kDa |
| 翻译后修饰 | Phosphorylated on serine and threonine residues in response to heregulin. Phosphorylated on tyrosine residues by TEK/TIE2. Phosphorylated on tyrosine residues in response to NTN1 signaling. Phosphorylation promotes stress granule disassembly during recovery after cellular stress (By similarity). Phosphorylated on tyrosine residues by PTK2/FAK1, and possibly also other kinases. Phosphorylation is enhanced by activation of receptor kinases. Tyrosine phosphorylation is essential for activation of down-stream protein kinases. |
检测图片 (2) Click the Picture to Zoom In
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ARG63205 anti-GRB7 antibody WB image
Western Blot: A431 lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63205 anti-GRB7 antibody at 2 µg/ml dilution.
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ARG63205 anti-GRB7 antibody WB image
Western Blot: 1). Mock transfection; 2) GRB7 (RC215362) expressing plasmid transfected HEK293 cell lysate standed with ARG63205 anti-GRB7 antibody
