ARG64038
anti-GPX4 antibody
anti-GPX4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Ferroptosis/Oxytosis Study antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GPX4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Pig |
| 应用 | IHC-P, IP, WB |
| 特异性 | This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GPX4 |
| 抗原物种 | Human |
| 抗原 | C-EEPLVIEKDLPHY |
| 偶联标记 | Un-conjugated |
| 別名 | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC 1.11.1.12; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P36969 Human Phospholipid hydroperoxide glutathione peroxidase, mitochondrial |
|---|---|
| 背景介绍 | Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq, Jul 2008] |
| 产品亮点 | Related products: GPX4 antibodies; GPX4 Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: Ferroptosis/Oxytosis Antibody Panel is launched Therapeutic strategies against PDAC |
| 研究领域 | Ferroptosis/Oxytosis Study antibody |
| 预测分子量 | 22 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG64038 anti-GPX4 antibody WB image
Western Blot: Human Testis lysate (35 µg protein in RIPA buffer) stained with ARG64038 anti-GPX4 (Isoform a and c) antibody at 0.1 µg/ml dilution.
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ARG64038 anti-GPX4 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Microwaved antigen retrieval with citrate buffer pH 6) stained with ARG64038 anti-GPX4 (Isoform a and c) antibody at 0.3 µg/ml dilution followed by HRP-staining.
