ARG43433
anti-GPR56 antibody
anti-GPR56 antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes GPR56 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1, kappa |
| 靶点名称 | GPR56 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human GPR56. |
| 偶联标记 | Un-conjugated |
| 別名 | BPPR; G-protein coupled receptor 56; C; GPR56 subunit alpha; BFPP; GPR56 NT; Protein TM7XN1; TM7XN1; GPR56 seven-transmembrane subunit; GPR56 extracellular subunit; N; GPR56 subunit beta; GPR56 7TM; GPR56; TM7LN4; GPR56 CT |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | U87-MG | ||||||
| 实际分子量 | ~ 75 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ADGRG1 |
| 全名 | adhesion G protein-coupled receptor G1 |
| 背景介绍 | This gene encodes a member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| 生物功能 | Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination (By similarity). Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12 (PubMed:22238662). Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche (By similarity). Plays a critical role in cancer progression by inhibiting VEGFA production threreby inhibiting angiogenesis through a signaling pathway mediated by PRKCA (PubMed:16757564, PubMed:21724588). Plays an essential role in testis development (By similarity). [ADGRG1 N-terminal fragment]: Plays a critical role in cancer progression by activating VEGFA production and angiogenesis through a signaling pathway mediated by PRKCA (PubMed:21724588). [UniProt] |
| 细胞定位 | Cell membrane; Multi-pass membrane protein. ADGRG1 N-terminal fragment: Secreted. ADGRG1 C-terminal fragment: Membrane raft. Note=Interaction with its ligand COL3A1 leads to the release of ADGRG1 NT from the membrane and triggers the association of ADGRG1 CT with lipid rafts. [UniProt] |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Autoproteolytically cleaved into 2 fragments; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1 CT) predominantly remain associated and non-covalently linked. Shedding to yield the secreted ADGRG1 N-terminal fragment seems to involve metalloprotease(s) (PubMed:22333914). N-glycosylated. Contains sialic acid residues. Ubiquitinated. Undergoes polyubiquitination upon activation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG43433 anti-GPR56 antibody WB image
Western blot: 20 µg of U87-MG whole cell lysate stained with ARG43433 anti-GPR56 antibody at 1:1000 dilution.
