ARG55822
anti-GPR56 antibody [1443CT377.65.29]
anti-GPR56 antibody [1443CT377.65.29] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes GPR56 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1443CT377.65.29 |
| 同位型 | IgG1, kappa |
| 靶点名称 | GPR56 |
| 抗原物种 | Human |
| 抗原 | Recombinant GPR56 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | BPPR; G-protein coupled receptor 56; C; GPR56 subunit alpha; BFPP; GPR56 NT; Protein TM7XN1; TM7XN1; GPR56 seven-transmembrane subunit; GPR56 extracellular subunit; N; GPR56 subunit beta; GPR56 7TM; GPR56; TM7LN4; GPR56 CT |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ADGRG1 |
| 全名 | adhesion G protein-coupled receptor G1 |
| 背景介绍 | This gene encodes a member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| 生物功能 | Involved in cell adhesion and probably in cell-cell interactions. Regulates the migration of neural precursor cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basemant membrane integrity and in cortical lamination. Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12. Isoforms show differences in receptor signaling, specifically in serum response element (SRE) transcriptional activation upon overexpression. Overexpression inhibits melanoma tumor growth and metastasis and, during melanoma progression, regulates VEGFA production and angiogenesis through PRKCA; unprocessed GPR56 is inhibiting and GPR56 NT is activating angiogenesis. Required for normal cortical development and regulation of neuroprogenitor cells proliferation. [UniProt] |
| 细胞定位 | Cell membrane; Multi-pass membrane protein |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Autoproteolytically cleaved into 2 fragments; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1 CT) predominantly remain associated and non-covalently linked. Shedding to yield the secreted ADGRG1 N-terminal fragment seems to involve metalloprotease(s) (PubMed:22333914). N-glycosylated. Contains sialic acid residues. Ubiquitinated. Undergoes polyubiquitination upon activation. |
检测图片 (1) Click the Picture to Zoom In
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ARG55822 anti-GPR56 antibody WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG55822 anti-GPR56 antibody at 1:2000 dilution.
