ARG65044
anti-GPR17 antibody
anti-GPR17 antibody for ICC/IF,Western blot and Human,Mouse
Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GPR17 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GPR17 |
| 抗原物种 | Human |
| 抗原 | C-EGKTNESSLSAKSE |
| 偶联标记 | Un-conjugated |
| 別名 | R12; P2Y-like receptor; G-protein coupled receptor 17; Uracil nucleotide/cysteinyl leukotriene receptor; UDP/CysLT receptor |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Mouse brain | ||||||
| 实际分子量 | ~ 41 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13304 Human Uracil nucleotide/cysteinyl leukotriene receptor Swiss-port # Q6NS65 Mouse Uracil nucleotide/cysteinyl leukotriene receptor |
|---|---|
| 基因名称 | GPR17 |
| 全名 | G protein-coupled receptor 17 |
| 生物功能 | Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia. [UniProt] |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 41 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG65044 anti-GPR17 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG65044 anti-GPR17 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65044 anti-GPR17 antibody WB image
Western blot: 35 µg of Mouse brain lysate (in RIPA buffer) stained with ARG65044 anti-GPR17 antibody at 3 µg/ml dilution and incubated at RT for 1 hour.
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ARG65044 anti-GPR17 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG65044 anti-GPR17 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
