ARG55054
anti-GOLPH3 antibody
anti-GOLPH3 antibody for ELISA,ICC/IF,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GOLPH3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GOLPH3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (14 aa) within the first 50 aa of Human GOLPH3. |
| 偶联标记 | Un-conjugated |
| 別名 | Mitochondrial DNA absence factor; Golgi phosphoprotein 3; GOPP1; Coat protein GPP34; MIDAS; Vps74; GPP34 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Rat Lung Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GOLPH3 |
| 全名 | golgi phosphoprotein 3 (coat-protein) |
| 背景介绍 | GOLPH3 Antibody: GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies. |
| 生物功能 | Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis. [UniProt] |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | Phosphorylated. |
检测图片 (2) Click the Picture to Zoom In
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ARG55054 anti-GOLPH3 antibody WB image
Western blot: rat lung tissue lysate stained with ARG55054 anti-GOLPH3 antibody at (A) 0.5 and (B) 1 ug/ml dilution.
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ARG55054 anti-GOLPH3 antibody ICC/IF image
Immunofluorescence: Rat Lung cells stained with ARG55054 anti-GOLPH3 antibody at 20 ug/ml dilution.
