ARG58816
anti-GNB1 antibody
anti-GNB1 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GNB1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov, Dog, Hm, Hrs, Mk, Rb, Zfsh |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GNB1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 2-42 of Human GNB1 (SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR). |
| 偶联标记 | Un-conjugated |
| 別名 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; Transducin beta chain 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GNB1 |
| 全名 | guanine nucleotide binding protein (G protein), beta polypeptide 1 |
| 背景介绍 | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| 生物功能 | Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. [UniProt] |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
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ARG58816 anti-GNB1 antibody ICC/IF image
Immunofluorescence: A549 cells were blocked with 10% goat serum and then stained with ARG58816 anti-GNB1 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG58816 anti-GNB1 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58816 anti-GNB1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58816 anti-GNB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG58816 anti-GNB1 antibody at 1 µg/ml dilution.
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ARG58816 anti-GNB1 antibody WB image
Western blot: Rat liver, Mouse cardiac muscle and HeLa lysates stained with ARG58816 anti-GNB1 antibody at 0.5 µg/ml dilution.
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ARG58816 anti-GNB1 antibody FACS image
Flow Cytometry: U937 cells were blocked with 10% normal goat serum and then stained with ARG58816 anti-GNB1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58816 anti-GNB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG58816 anti-GNB1 antibody at 1 µg/ml dilution.
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ARG58816 anti-GNB1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer stained with ARG58816 anti-GNB1 antibody at 1 µg/ml dilution.
