ARG64659
anti-GNAT3 antibody
anti-GNAT3 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GNAT3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog |
| 应用 | IHC-P |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GNAT3 |
| 抗原物种 | Human |
| 抗原 | C-KNQFLDLNLKKEDKE |
| 偶联标记 | Un-conjugated |
| 別名 | GDCA; Guanine nucleotide-binding protein G(t) subunit alpha-3; Gustducin alpha-3 chain |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # A8MTJ3 Human Guanine nucleotide-binding protein G(t) subunit alpha-3 |
|---|---|
| 基因名称 | GNAT3 |
| 全名 | guanine nucleotide binding protein, alpha transducing 3 |
| 生物功能 | Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity. [UniProt] |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane. |
检测图片 (1) Click the Picture to Zoom In
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ARG64659 anti-GNAT3 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Small Intestine. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64659 anti-GNAT3 antibody at 3.8 µg/ml dilution followed by AP-staining.
