ARG58910
anti-GNAI2 antibody
anti-GNAI2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes GNAI2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Pig |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_002061.1; NP_001159897.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GNAI2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from the internal region of Human GNAI2. (NP_002061.1; NP_001159897.1) (C-PEYTGANKYDE) |
| 偶联标记 | Un-conjugated |
| 別名 | GIP; GNAI2B; H_LUCA15.1; H_LUCA16.1; Guanine nucleotide-binding protein G(i) subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P04899 Human Guanine nucleotide-binding protein G(i) subunit alpha-2 |
|---|---|
| 基因名称 | GNAI2 |
| 全名 | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 |
| 背景介绍 | The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| 生物功能 | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. Isoform sGi2: Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool. [UniProt] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell membrane. Membrane; Lipid-anchor. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. [UniProt] |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | (Microbial infection) Deamidated at Gln-205 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58910 anti-GNAI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG58910 anti-GNAI2 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG58910 anti-GNAI2 antibody WB image
Western blot: 35 µg of Human peripheral blood lymphocyte lysates (in RIPA buffer) stained with ARG58910 anti-GNAI2 antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
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ARG58910 anti-GNAI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG58910 anti-GNAI2 antibody at 5 µg/ml dilution followed by AP-staining.
