ARG56690

anti-GM-CSF antibody

anti-GM-CSF antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes GM-CSF
反应物种 Hu
应用 ELISA, IHC-P, Neut, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GM-CSF
抗原物种 Human
抗原 E.coli derived Recombinant Human GM-CSF.
(MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE)
偶联标记 Un-conjugated
別名 GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56800 as a detection antibody
IHC-P0.25 µg/ml
Neut0.13 - 0.20 µg/ml (To yield [ND50] of the biological activity of hGM - CSF (1.0 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1437 Human CSF2

Swiss-port # P04141 Human Granulocyte-macrophage colony-stimulating factor

基因名称 CSF2
全名 colony stimulating factor 2 (granulocyte-macrophage)
背景介绍 The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
生物功能 Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt]
产品亮点 Related products:
GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
预测分子量 16 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG56690 anti-GM-CSF antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56690 anti-GM-CSF antibody concentration is 0.25 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56690 anti-GM-CSF antibody WB image

    Western blot: 250 - 0.24 ng of Human GM-CSF stained with ARG56690 anti-GM-CSF antibody, under reducing conditions.

  • ARG56690 anti-GM-CSF antibody WB image

    Western blot: 250 - 0.24 ng of Human GM-CSF stained with ARG56690 anti-GM-CSF antibody, under non-reducing conditions.

  • ARG56690 anti-GM-CSF antibody standard curve image

    Sandwich ELISA: ARG56690 anti-GM-CSF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56800 anti-GM-CSF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.