ARG58895
anti-GLI1 antibody
anti-GLI1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GLI1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GLI1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 200-300 of Human GLI1 (NP_001153517.1). |
| 偶联标记 | Un-conjugated |
| 別名 | GLI; Glioma-associated oncogene; Zinc finger protein GLI1; Oncogene GLI |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Rat brain | ||||||||
| 实际分子量 | 160 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GLI1 |
| 全名 | GLI family zinc finger 1 |
| 背景介绍 | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| 生物功能 | Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable). Isoform 2: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration. [UniProt] |
| 细胞定位 | Cytoplasm, Nucleus, Nucleus,. [UniProt] |
| 预测分子量 | 118 kDa |
| 翻译后修饰 | Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58895 anti-GLI1 antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG58895 anti-GLI1 antibody at 1:100 dilution.
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ARG58895 anti-GLI1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis stained with ARG58895 anti-GLI1 antibody at 1:100 dilution.
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ARG58895 anti-GLI1 antibody WB image
Western blot: 25 µg of Rat brain lysate stained with ARG58895 anti-GLI1 antibody at 1:1000 dilution.
