ARG59003
anti-GIT1 antibody
anti-GIT1 antibody for Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GIT1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GIT1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 366-395 of Human GIT1. |
| 偶联标记 | Un-conjugated |
| 別名 | G protein-coupled receptor kinase-interactor 1; Cool-associated and tyrosine-phosphorylated protein 1; ARF GTPase-activating protein GIT1; CAT-1; CAT1; GRK-interacting protein 1; ARF GAP GIT1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | U-251 MG |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q68FF6 Mouse ARF GTPase-activating protein GIT1 Swiss-port # Q9Y2X7 Human ARF GTPase-activating protein GIT1 |
|---|---|
| 基因名称 | GIT1 |
| 全名 | G protein-coupled receptor kinase interacting ArfGAP 1 |
| 生物功能 | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin. [UniProt] |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59003 anti-GIT1 antibody WB image
Western blot: 20 µg of U-251 MG cell lysate stained with ARG59003 anti-GIT1 antibody at 1:1000 dilution.
