ARG63901
anti-GEF5 antibody
anti-GEF5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes GEF5 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GEF5 |
| 抗原物种 | Human |
| 抗原 | C-DKEIDQNSQQEE |
| 偶联标记 | Un-conjugated |
| 別名 | P60; TIM1; Oncogene TIM; Rho guanine nucleotide exchange factor 5; TIM; GEF5; Ephexin-3; p60 TIM; Transforming immortalized mammary oncogene; Guanine nucleotide regulatory protein TIM |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q12774 Human Rho guanine nucleotide exchange factor 5 |
|---|---|
| 背景介绍 | Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 177 kDa |
| 翻译后修饰 | Activation of SRC induces tyrosine phosphorylation of ARHGEF5. |
检测图片 (2) Click the Picture to Zoom In
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ARG63901 anti-GEF5 antibody WB image
Western Blot: HeLa Lysate (35 µg protein in RIPA buffer) stained with ARG63901 anti-GEF5 antibody at 0.1 µg/ml dilution.
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ARG63901 anti-GEF5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63901 anti-GEF5 antibody at 3.75 µg/ml dilution followed by AP-staining.
