ARG53932
anti-GCPII / PSMA antibody [GCP-05] (PE)
anti-GCPII / PSMA antibody [GCP-05] (PE) for Flow cytometry and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [GCP-05] recognizes GCPII / PSMA |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | GCP-05 |
| 同位型 | IgG1 |
| 靶点名称 | GCPII / PSMA |
| 抗原物种 | Human |
| 抗原 | amino acids 44-750 of human GCPII |
| 偶联标记 | PE |
| 別名 | FOLH1; Folate Hydrolase 1; NAALAD1; GCPII; PSMA; PSM; Glutamate Carboxypeptidase 2; GCP2; FOLH; N-Acetylated-Alpha-Linked Acidic Dipeptidase I; Pteroylpoly-Gamma-Glutamate Carboxypeptidase; Folylpoly-Gamma-Glutamate Carboxypeptidase; Cell Growth-Inhibiting Gene 27 Protein; Membrane Glutamate Carboxypeptidase; Glutamate Carboxypeptidase II; Glutamate Carboxylase II; EC 3.4.17.21; NAALADase I; NAALAdase; FGCP; MGCP; Folate Hydrolase (Prostate-Specific Membrane Antigen) 1; N-Acetylated Alpha-Linked Acidic Dipeptidase 1; Prostate-Specific Membrane Antigen |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FOLH1 |
| 全名 | folate hydrolase (prostate-specific membrane antigen) 1 |
| 背景介绍 | This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. |
| 生物功能 | Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. |
| 细胞定位 | Cell membrane, Cytoplasm, Membrane |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | Glycoprotein, Phosphoprotein |
检测图片 (1) Click the Picture to Zoom In
-
ARG53932 anti-GCPII / PSMA antibody [GCP-05] (PE) FACS image
Flow Cytometry: Separation of LNCaP cells (red) from SP2 cells (blue). Cells were stained with ARG53932 anti-GCPII / PSMA antibody [GCP-05] (PE) at 3 µg/ml dilution.
克隆号文献
Gold nano-popcorn-based targeted diagnosis, nanotherapy treatment, and in situ monitoring of photothermal therapy response of prostate cancer cells using surface-enhanced Raman spectroscopy.
ICC/IF / Human
Expression of glutamate carboxypeptidase II in human brain.
Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding.
Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.
