ARG57117
anti-GCLM antibody [2D12]
anti-GCLM antibody [2D12] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [2D12] recognizes GCLM |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2D12 |
| 同位型 | IgG1, kappa |
| 靶点名称 | GCLM |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-274 of Human GCLM |
| 偶联标记 | Un-conjugated |
| 別名 | GCS light chain; Gamma-ECS regulatory subunit; Glutamate--cysteine ligase regulatory subunit; GLCLR; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P48507 Human Glutamate--cysteine ligase regulatory subunit |
|---|---|
| 基因名称 | GCLM |
| 全名 | glutamate-cysteine ligase, modifier subunit |
| 背景介绍 | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
| 预测分子量 | 31 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57117 anti-GCLM antibody [2D12] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57117 anti-GCLM antibody [2D12] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57117 anti-GCLM antibody [2D12] WB image
Western blot: 40 µg of HeLa cell lysate stained with ARG57117 anti-GCLM antibody [2D12] at 1:3000.
