ARG58032

anti-GCLC antibody

anti-GCLC antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes GCLC
反应物种 Hu, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 GCLC
抗原物种 Human
抗原 Partial recombinant protein corresponding to aa. 437-637 of Human GCLC.
偶联标记 Un-conjugated
別名 GCS heavy chain; Gamma-glutamylcysteine synthetase; Gamma-ECS; GCL; GLCL; GCS; Glutamate--cysteine ligase catalytic subunit; EC 6.3.2.2; GLCLC

应用说明

应用建议
应用 推荐稀释比
IHC-P1 - 5 µg/ml
WB0.5 - 1 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 72 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.025% Sodium azide and 2.5% BSA.
抗菌剂 0.025% Sodium azide
稳定剂 2.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 25283 Rat GCLC

GeneID: 2729 Human GCLC

Swiss-port # P19468 Rat Glutamate--cysteine ligase catalytic subunit

Swiss-port # P48506 Human Glutamate--cysteine ligase catalytic subunit

基因名称 GCLC
全名 glutamate-cysteine ligase, catalytic subunit
背景介绍 Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
预测分子量 73 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG58032 anti-GCLC antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG58032 anti-GCLC antibody.

  • ARG58032 anti-GCLC antibody WB image

    Western blot: 1) Rat brain, 2) Rat heart, 3) HeLa, 4) Rat PC12, 5) Rat NRK, 6) Mouse HEPA and 7) A549 cell lysates stained with ARG58032 anti-GCLC antibody.

  • ARG58032 anti-GCLC antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG58032 anti-GCLC antibody.

  • ARG58032 anti-GCLC antibody WB image

    Western blot: 0.5 ng of recombinant Human protein stained with ARG58032 anti-GCLC antibody.