ARG45887
anti-GCH1 antibody
anti-GCH1 antibody for Western blot,Flow cytometry and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GCH1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GCH1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human GCH1. |
| 偶联标记 | Un-conjugated |
| 別名 | GCH1; DYT14; HPABH4B; GTPCH1; GCH; GTP cyclohydrolase 1; GTP-CH-1; DYT5; GTP cyclohydrolase I; GTP-CH-I; DYT5a; EC 3.5.4.16 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 30 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GCH1 |
| 全名 | GTP Cyclohydrolase 1 |
| 背景介绍 | This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008] |
| 生物功能 | Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown. [UniProt] |
| 细胞定位 | Cytoplasm; Nucleus. [UniProt] |
| 预测分子量 | 28 kDa |
| 翻译后修饰 | Phosphorylated by casein kinase II at Ser-81 in HAECs during oscillatory shear stress; phosphorylation at Ser-81 results in increased enzyme activity. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG45887 anti-GCH1 antibody WB image
Western blot: 293T stained with ARG45887 anti-GCH1 antibody at 0.5 μg/ml dilution.
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ARG45887 anti-GCH1 antibody FACS image
Flow Cytometry: Jurkat stained with ARG45887 anti-GCH1 antibody at 1 µg/10^6 cells dilution.
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ARG45887 anti-GCH1 antibody WB image
Western blot: Rat liver stained with ARG45887 anti-GCH1 antibody at 0.5 μg/ml dilution.
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ARG45887 anti-GCH1 antibody WB image
Western blot: Mouse liver stained with ARG45887 anti-GCH1 antibody at 0.5 μg/ml dilution.
