ARG55113
anti-GATA3 antibody
anti-GATA3 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GATA3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GATA3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (17 aa) within aa. 340-390 of Human GATA3 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; HDR; HDRS |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human Brain Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P23771 Human Trans-acting T-cell-specific transcription factor GATA-3 Swiss-port # P23772 Mouse Trans-acting T-cell-specific transcription factor GATA-3 |
|---|---|
| 基因名称 | GATA3 |
| 全名 | GATA binding protein 3 |
| 背景介绍 | GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer. |
| 生物功能 | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. [UniProt] |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 48 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG55113 anti-GATA3 antibody WB image
Western blot: human brain tissue lysate stained with ARG55113 anti-GATA3 antibody at (A) 1 and (B) 2 ug/ml dilution.
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ARG55113 anti-GATA3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human airway tissue stained with ARG55113 anti-GATA3 antibody. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0).
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ARG55113 anti-GATA3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded virus infected Mouse lung tissue stained with ARG55113 anti-GATA3 antibody (green) at 1:100 dilution. Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0).
