ARG62345

anti-GAPDH antibody [GA1R]

anti-GAPDH antibody [GA1R] for Dot blot,ELISA,ICC/IF,Western blot and Human,Mouse,Rat,Chicken,E. coli,Hamster,Insect,Pig,Rabbit,S. cerevisiae

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody
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publication_link 参考文献4

概述

产品描述

Mouse Monoclonal antibody [GA1R] recognizes GAPDH

反应物种 Hu, Ms, Rat, Chk, E. coli, Hm, Insect, Pig, Rb, S. cerevisiae
应用 Dot, ELISA, ICC/IF, WB
特异性 Recognizes native and denatured forms of GAPDH (∼37kDa).
宿主 Mouse
克隆 Monoclonal
克隆号 GA1R
同位型 IgG1
靶点名称 GAPDH
抗原 Recombinant GAPDH
偶联标记 Un-conjugated
別名 Glyceraldehyde-3-phosphate dehydrogenase; GAPD; HEL-S-162eP; G3PD; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; EC 2.6.99.-; EC 1.2.1.12

应用说明

应用建议
应用 推荐稀释比
DotAssay-dependent
ELISAAssay-dependent
ICC/IFAssay-dependent
WB1:1000-1:10000
应用说明 The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化说明 Protein A affinity chromatography from mouse ascites fluid.
缓冲液 10mM PBS (pH 7.2) and 0.05% Sodium azide
抗菌剂 0.05% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 100009074 Rabbit GAPDH

GeneID: 14433 Mouse GAPDH

GeneID: 24383 Rat GAPDH

基因名称 Gapdh
全名 glyceraldehyde-3-phosphate dehydrogenase
背景介绍 GAPDH protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
生物功能 GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. [UniProt]
研究领域 Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody
预测分子量 36 kDa
翻译后修饰 S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.
ISGylated.
Sulfhydration at Cys-152 increases catalytic activity.
Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.

检测图片 (3) Click the Picture to Zoom In

  • ARG62345 anti-GAPDH antibody [GA1R] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG62345 anti-GAPDH antibody [GA1R] (green) at 1:50 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG62345 anti-GAPDH antibody [GA1R] WB image

    Western Blot: 5 μg/lane tissue lysates from (1) human, (2) mouse, (3) rat, (4) rabbit, (5) chicken, and (6) hamster stained with ARG62345 anti-GAPDH antibody [GA1R] at 1:2000 (0.5 μg/mL) dilution

  • ARG62345 anti-GAPDH antibody [GA1R] WB image

    Western Blot: (1) Escherichia coli strain BL21, (2) Sf9 insect, and (3) Saccharomyces cerevisiae stained with ARG62345 anti-GAPDH antibody [GA1R] at 1:2000 dilution.

客户反馈

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anti-GAPDH antibody [GA1R]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:50

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

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anti-GAPDH antibody [GA1R]

Application:WB

Sample:293T, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-GAPDH antibody [GA1R]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Metabolic Remodeling by the PD-L1 Inhibitor BMS-202 Significantly Inhibits Cell Malignancy in Human Glioblastomas

WB / Human

Xueou Yang et al.
,  (2023)

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Codon optimization of the synthetic 3-ketosphinganine reductase (3KSR) protein for enhancing sphingolipid biosynthetic enzyme expression

WB / Saccharomyces cerevisiae

Hyun-Ju Um et al.
Molecular & Cellular Toxicology,  (2021)

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A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells.

WB / E. coli

Li Xie et al.
Nucleic Acids Res.,  (2020)

publication_link

 

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Selenoprotein M stimulates the proliferative and metastatic capacities of renal cell carcinoma through activating the PI3K/AKT/mTOR pathway.

WB / Human

Jiang Hao et al.
Cancer Med.,  (2019)

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