ARG10112

anti-GAPDH antibody [6C5]

anti-GAPDH antibody [6C5] for ELISA,ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,African green monkey,Baboon,Cat,Chicken,Dog,Fish,Hamster,Monkey,Pig,Rabbit,Xenopus laevis,Zebrafish

Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody
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概述

产品描述

Mouse Monoclonal antibody [6C5] recognizes GAPDH

反应物种 Hu, Ms, Rat, AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Mk, Pig, Rb, Xenopus laevis, Zfsh
预测物种 Gpig, Hrs, Xenopus tropicalis
不反应物种 Bov, Goat, S. cerevisiae
应用 ELISA, ICC/IF, IHC-Fr, WB
特异性 This antibody is specific for GAPDH of Human, Porcine, Canine, Rabbit, Cat, Rat, Mouse, Fish.
宿主 Mouse
克隆 Monoclonal
克隆号 6C5
同位型 IgG1
靶点名称 GAPDH
抗原物种 Rabbit
抗原 Rabbit muscle GAPDH
偶联标记 Un-conjugated
別名 Glyceraldehyde-3-phosphate dehydrogenase; GAPD; HEL-S-162eP; G3PD; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; EC 2.6.99.-; EC 1.2.1.12

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
ICC/IF5 µg/ml
IHC-Fr1:500
WB1:1000 - 1:10000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HeLa; NTERA-2; A-431; HepG2; MCF-; NIH 3T3; PC-12; COS-7

属性

形式 Liquid
纯化 Protein A affinity purified.
缓冲液 PBS (pH 7.4) and 0.09% Sodium azide
抗菌剂 0.09% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 100009074 Rabbit GAPDH

GeneID: 14433 Mouse GAPDH

GeneID: 24383 Rat GAPDH

基因名称 GAPDH
全名 glyceraldehyde-3-phosphate dehydrogenase
背景介绍 GAPDH protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
生物功能 GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30001 Tag Internal Control Antibody Duo (GFP, GAPDH)
ARG30002 Tag Internal Control Antibody Duo (His tag, GAPDH)
ARG30061 Tag Internal Control Antibody Duo (dsRed, GAPDH)
ARG30062 Tag Internal Control Antibody Duo (YFP, GAPDH)
ARG30251 NFkB nuclear translocation Antibody Panel
ARG30259 Loading Controls for Cytoplasmic / Nuclear Fractions Antibody Panel
ARG30267 Organelle Marker, Cytoplasm, Nucleus Antibody Duo (Histone, GAPDH)
ARG30270 Loading Control Antibody Panel (Actin, beta Tublin, Histone H3, GAPDH)
ARG30331 NLRP3 Inflammasome Antibody Panel
ARG30332 NLRC4 Inflammasome Antibody Panel
Related products:
GAPDH antibodies; GAPDH Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
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研究领域 Cancer antibody; Controls and Markers antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for Cytoplasmic Fractions; Organelle Marker antibody for Cytoplasm; Autophagy Study antibody
预测分子量 36 kDa
翻译后修饰 S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.
ISGylated.
Sulfhydration at Cys-152 increases catalytic activity.
Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.

检测图片 (4) Click the Picture to Zoom In

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) HepG2 3) rat brain 4) rat heart 5) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:2000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10112 anti-GAPDH antibody [6C5] (green) at 1:200 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) U87-MG 2) MCF-7 3) A549 4) DU145 5) SW480 6) rat brain 7) rat stomach 8) rat ovary stained with ARG10112 anti-GAPDH antibody [6C5] at 1:5000 dilution.

  • ARG10112 anti-GAPDH antibody [6C5] WB image

    Western blot: 1) MCF-7 2) DU-145 3) A549 4) H1299 5) HCT116 6) HepG2 7) HUVEC stained with ARG10112 anti-GAPDH antibody [6C5] at 1:1000 dilution.

客户反馈

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anti-GAPDH antibody [6C5]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

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anti-GAPDH antibody [6C5]

Application:WB

Sample:HaCaT cell

Sample Loading Amount:40μg

Primary Antibody Dilution Factor:1:10,000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

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Restraint stress promotes lung cancer xenograft growth via the IL2-Ras-Erk pathway

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A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage

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SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis

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Uric acid mitigates cognitive deficits via transcription factor EB - mediated microglial autophagy in mice models of Alzheimer's disease

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Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH

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Genetic labeling reveals spatial and cellular expression pattern of neuregulin 1 in mouse brain

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Cell Biosci.,  (2023)

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Erucic acid improves the progress of pregnancy complicated with systemic lupus erythematosus by inhibiting the effector function of CD8+ T cells

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PPAR- γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1 α/ Δψ m Pathways by Inhibiting Pyroptosis

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Zhencheng Feng et al.
PPAR Res,  (2023)

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PPARγ activation suppresses chondrocyte ferroptosis through mitophagy in osteoarthritis

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Discovery of the First Subnanomolar PPARα/δ Dual Agonist for the Treatment of Cholestatic Liver Diseases

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Genetic labeling reveals cellular expression pattern of neuregulin 1 in mouse forebrain

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Qi-Tai-Suan, an oleanolic acid derivative, ameliorates ischemic heart failure via suppression of cardiac apoptosis, inflammation and fibrosis

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SCM-198 Prevents Endometriosis by Reversing Low Autophagy of Endometrial Stromal Cell via Balancing ERα and PR Signals

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Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

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Chinese Journal of Immunology.,  (2020)

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Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

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SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways.

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Acta Biochim Biophys Sin.,  (2019)

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