ARG10735
anti-GAP43 antibody
anti-GAP43 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GAP43 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Hrs, Pig |
| 预测物种 | Chk |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GAP43 |
| 抗原物种 | Mouse |
| 抗原 | C-terminal peptide of Mouse and Rat GAP43, which is KEDPEADQEHA, with an N-terminal Cys added to allow chemical coupling to KLH carrier protein. |
| 偶联标记 | Un-conjugated |
| 別名 | pp46; Growth-associated protein 43; B-50; Neuromodulin; PP46; Axonal membrane protein GAP-43; Neural phosphoprotein B-50 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Gap43 |
| 全名 | growth associated protein 43 |
| 背景介绍 | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2. |
检测图片 (4) Click the Picture to Zoom In
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ARG10735 anti-GAP43 antibody ICC/IF image
Immunocytochemistry: Rat mixed neuron-glial cultures stained with ARG10735 anti-GAP43 antibody (red). Blue is DNA staining.
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ARG10735 anti-GAP43 antibody ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10735 anti-GAP43 antibody (green) at 1:2000 dilution and costained with Mouse mAb to vimentin (red) at 1:2000 dilution. DAPI (blue) for nuclear staining.
The GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.
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ARG10735 anti-GAP43 antibody WB image
Western blot: Homogenate of cow cerebellum stained with ARG10735 anti-GAP43 antibody.
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ARG10735 anti-GAP43 antibody WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord, SH-SY5Y and C6 cell lysates stained with ARG10735 anti-GAP43 antibody (green) at 1:20000 dilution.
The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a Rat glioma cell line and do not express GAP43 protein.
