ARG54931
anti-GADD45 alpha antibody
anti-GADD45 alpha antibody for Flow cytometry,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GADD45A |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Bov |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GADD45 alpha |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human GADD45 alpha. |
| 偶联标记 | Un-conjugated |
| 別名 | Growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT1; GADD45; DNA damage-inducible transcript 1 protein; DDIT-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Y79 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P24522 Human Growth arrest and DNA damage-inducible protein GADD45 alpha |
|---|---|
| 基因名称 | GADD45A |
| 全名 | growth arrest and DNA-damage-inducible, alpha |
| 背景介绍 | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010] |
| 生物功能 | In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. [UniProt] |
| 细胞定位 | Nucleus. |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 18 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54931 anti-GADD45 alpha antibody WB image
Western blot: 35 µg of Y79 cell lysate stained with ARG54931 anti-GADD45 alpha antibody.
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ARG54931 anti-GADD45 alpha antibody FACS image
Flow Cytometry: K562 cells stained with ARG54931 anti-GADD45 alpha antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
