ARG22242
anti-GABAB Receptor 2 antibody [S81-2]
anti-GABAB Receptor 2 antibody [S81-2] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [S81-2] recognizes GABAB Receptor 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Detects ~105kDa. No cross-reactivity against GABA(B)R1. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S81-2 |
| 同位型 | IgG1 |
| 靶点名称 | GABAB Receptor 2 |
| 抗原物种 | Rat |
| 抗原 | Fusion protein around aa. 861-912 of Rat GABA B Receptor 2 |
| 偶联标记 | Un-conjugated |
| 別名 | HRIHFB2099; GABA-B receptor 2; GABA-B-R2; GPRC3B; GABA-BR2; G-protein coupled receptor 51; GPR51; GABABR2; Gamma-aminobutyric acid type B receptor subunit 2; HG20; Gb2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Gabbr2 |
| 全名 | gamma-aminobutyric acid (GABA) B receptor 2 |
| 背景介绍 | The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010] |
| 生物功能 | Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. [UniProt] |
| 细胞定位 | Cell Junction, Cell membrane, postsynaptic cell membrane, Synapse |
| 预测分子量 | 106 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG22242 anti-GABAB Receptor 2 antibody [S81-2] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary antibody: ARG22242 anti-GABAB Receptor 2 antibody [S81-2] at 1:100 for 60 min at RT. Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Primary antibody (D) Composite.
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ARG22242 anti-GABAB Receptor 2 antibody [S81-2] WB image
Western blot: 10 µg of Rat Brain Membrane. Block: 5% milk. Primary antibody: ARG22242 anti-GABAB Receptor 2 antibody [S81-2] at 1:1000 for 1 hour at RT. Secondary antibody: Goat anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT.
