ARG51770
anti-GAB1 phospho (Tyr627) antibody
anti-GAB1 phospho (Tyr627) antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes GAB1 phospho (Tyr627) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | GAB1 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1. |
| 偶联标记 | Un-conjugated |
| 別名 | Growth factor receptor bound protein 2-associated protein 1; GRB2-associated binder 1; GRB2-associated-binding protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GAB1 |
| 全名 | GRB2-associated binding protein 1 |
| 背景介绍 | The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. |
| 生物功能 | Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling. |
检测图片 (2) Click the Picture to Zoom In
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ARG51770 anti-GAB1 phospho (Tyr627) antibody WB image
Western blot: Extracts from 293 cells untreated or treated with EGF stained with ARG51770 anti-GAB1 phospho (Tyr627) antibody.
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ARG51770 anti-GAB1 phospho (Tyr627) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51770 anti-GAB1 phospho (Tyr627) antibody.
