ARG41740
anti-G3BP1 antibody
anti-G3BP1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes G3BP1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | G3BP1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 131-330 of Human G3BP1 (NP_938405.1). |
| 偶联标记 | Un-conjugated |
| 別名 | G3BP; hDH VIII; G3BP-1; GAP SH3 domain-binding protein 1; EC 3.6.4.13; EC 3.6.4.12; HDH-VIII; ATP-dependent DNA helicase VIII; Ras GTPase-activating protein-binding protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | A431 | ||||||||
| 实际分子量 | ~ 68 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P97855 Mouse Ras GTPase-activating protein-binding protein 1 Swiss-port # Q13283 Human Ras GTPase-activating protein-binding protein 1 |
|---|---|
| 基因名称 | G3BP1 |
| 全名 | GTPase activating protein (SH3 domain) binding protein 1 |
| 背景介绍 | This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Cytoplasm, Stress granule. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells (By similarity). The unphosphorylated form is recruited to stress granules. HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610). [UniProt] |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. Arg-435 is dimethylated, probably to asymmetric dimethylarginine. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41740 anti-G3BP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG41740 anti-G3BP1 antibody at 1:100 dilution.
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ARG41740 anti-G3BP1 antibody WB image
Western blot: 25 µg of A431 cell lysate stained with ARG41740 anti-G3BP1 antibody at 1:1000 dilution.
