ARG56678

anti-G-CSF antibody

anti-G-CSF antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes G-CSF
反应物种 Hu
应用 ELISA, IHC-P, Neut, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 G-CSF
抗原物种 Human
抗原 E.coli derived Recombinant Human G-CSF.
(TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP)
偶联标记 Un-conjugated
別名 Granulocyte colony-stimulating factor; Lenograstim; C17orf33; GCSF; G-CSF; Filgrastim; Pluripoietin; CSF3OS

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56788 as a detection antibody
IHC-P0.25 µg/ml
Neut0.04 - 0.06 µg/ml (To yield [ND50] of the biological activity of hG - CSF (0.5 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1440 Human CSF3

Swiss-port # P09919 Human Granulocyte colony-stimulating factor

基因名称 CSF3
全名 colony stimulating factor 3 (granulocyte)
背景介绍 The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
生物功能 Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. [UniProt]
预测分子量 22 kDa
翻译后修饰 O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

检测图片 (4) Click the Picture to Zoom In

  • ARG56678 anti-G-CSF antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human angiosarcoma. The recommended ARG56678 anti-G-CSF antibody concentration is 0.25 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56678 anti-G-CSF antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human angiosarcoma. The recommended ARG56678 anti-G-CSF antibody concentration is 0.25 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56678 anti-G-CSF antibody WB image

    Western blot: 250 - 0.24 ng of Human G-CSF stained with ARG56678 anti-G-CSF antibody, under non-reducing conditions.

  • ARG56678 anti-G-CSF antibody standard curve image

    Sandwich ELISA: ARG56678 anti-G-CSF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56788 anti-G-CSF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.