ARG64623
anti-Frizzled 2 antibody
anti-Frizzled 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Frizzled 2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Frizzled 2 |
| 抗原物种 | Human |
| 抗原 | RYATLEHPFHCPR |
| 偶联标记 | Un-conjugated |
| 別名 | fzE2; FzE2; Fz2; Frizzled-2; hFz2; Fz-2; fz-2 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 64 kDa |
| 翻译后修饰 | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
检测图片 (4) Click the Picture to Zoom In
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ARG64623 anti-Frizzled 2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64623 anti-Frizzled 2 antibody (green) at 10 µg/ml dilution for 1 hour. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64623 anti-Frizzled 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64623 anti-Frizzled 2 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64623 anti-Frizzled 2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Thyroid Gland. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64623 anti-Frizzled 2 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64623 anti-Frizzled 2 antibody WB image
Western blot: 35 µg of Human heart (A) and Human placenta (B) (in RIPA buffer) stained with ARG64623 anti-Frizzled 2 antibody at 0.03 µg/ml (A) and 0.1 µg/ml (B) dilutions and incubated at RT for 1 hour.
