ARG58327
anti-Frataxin antibody
anti-Frataxin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Frataxin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Frataxin |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 42-210 of Human Frataxin (NP_000135.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Frataxin; Friedreich ataxia protein; d-FXN; Frataxin, mitochondrial; FRDA; i-FXN; m56-FXN; CyaY; m78-FXN; FA; Fxn; 56-210; FARR; X25; 78-210; m81-FXN; EC 1.16.3.1; 81-210 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Raji | ||||||||
| 实际分子量 | 14 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FXN |
| 全名 | frataxin |
| 背景介绍 | This nuclear gene encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009] |
| 生物功能 | Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1. [UniProt] |
| 细胞定位 | Cytoplasm, Mitochondrion. [UniProt] |
| 预测分子量 | 23 kDa |
| 翻译后修饰 | Processed in two steps by mitochondrial processing peptidase (MPP). MPP first cleaves the precursor to intermediate form and subsequently converts the intermediate to yield frataxin mature form (frataxin(81-210)) which is the predominant form. The additional forms, frataxin(56-210) and frataxin(78-210), seem to be produced when the normal maturation process is impaired; their physiological relevance is unsure. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58327 anti-Frataxin antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG58327 anti-Frataxin antibody.
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ARG58327 anti-Frataxin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse heart stained with ARG58327 anti-Frataxin antibody at 1:100 dilution.
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ARG58327 anti-Frataxin antibody WB image
Western blot: 25 µg of Raji cell lysate stained with ARG58327 anti-Frataxin antibody at 1:1000 dilution.
