ARG55493

anti-Flotillin 1 antibody

anti-Flotillin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Signaling Transduction antibody
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概述

产品描述 Rabbit Polyclonal antibody recognizes Flotillin 1
反应物种 Hu, Ms, Rat
预测物种 Rat
应用 ICC/IF, IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Flotillin 1
抗原物种 Human
抗原 Recombinant protein of Human FLOT1 (NP_005794.1)
偶联标记 Un-conjugated
別名 Flotillin-1

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
IPAssay-dependent
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10211 Human FLOT1

GeneID: 14251 Mouse FLOT1

GeneID: 64665 Rat FLOT1

基因名称 FLOT1
全名 flotillin 1
背景介绍 Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008]
生物功能 May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. [UniProt]
研究领域 Cell Biology and Cellular Response antibody; Signaling Transduction antibody
预测分子量 47 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG55493 anti-Flotillin 1 antibody WB image

    Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG55493 anti-Flotillin 1 antibody at 1:1000 dilution.

  • ARG55493 anti-Flotillin 1 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55493 anti-Flotillin 1 antibody (green). Up: Primary antibodies. Bottom: Merge with DAPI (blue).

客户反馈

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anti-Flotillin 1 antibody

Application:WB

Sample:HeLa and 293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC