ARG62491
anti-Filaggrin antibody [FLG01]
anti-Filaggrin antibody [FLG01] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
Controls and Markers antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [FLG01] recognizes Filaggrin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-Fr, IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | FLG01 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Filaggrin |
| 抗原 | Recombinant full length protein. |
| 偶联标记 | Un-conjugated |
| 別名 | ATOD2; Filaggrin |
应用说明
| 应用说明 | FACS: 1µg for 106 cells IHC-P: 2 - 4 µg/ml IHC-Fr: 1/200 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FLG |
| 全名 | filaggrin |
| 背景介绍 | The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009] |
| 生物功能 | Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. [UniProt] |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 435 kDa |
| 翻译后修饰 | Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate. Undergoes deimination of some arginine residues (citrullination). |
克隆号文献
Highly rapid and efficient conversion of human fibroblasts to keratinocyte-like cells.
Papillary fibroblasts differentiate into reticular fibroblasts after prolonged in vitro culture.
IHC / Human
Effects of niacin restriction on sirtuin and PARP responses to photodamage in human skin.
IHC-P / Human
Characterization of a unique technique for culturing primary adult human epithelial progenitor/stem cells.
ICC/IF / Human
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
ICC/IF / Human
Establishment and characterization of immortalized human gingival keratinocyte cell lines.
FACS / Human
