ARG56127

anti-Fibronectin antibody [FN12-8]

anti-Fibronectin antibody [FN12-8] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Inhibition assay,Western blot and Bovine,Human

概述

产品描述

Mouse Monoclonal antibody [FN12-8] recognizes Fibronectin

反应物种 Hu, Bov
应用 IHC-Fr, IHC-P, Inhib, WB
特异性 This antibody recognizes an epitope in the cell-binding domain of Human and Bovine fibronectin and can inhibit cell adhesion.
宿主 Mouse
克隆 Monoclonal
克隆号 FN12-8
同位型 IgG1
靶点名称 Fibronectin
抗原物种 Human
抗原 Human plasma fibronectin
偶联标记 Un-conjugated
全名 fibronectin 1
別名 ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

应用说明

应用建议
应用 推荐稀释比
IHC-Fr1 - 10 µg/ml
IHC-P1 - 10 µg/ml
Inhib0.1 - 1.0 µg/ml on 10 µg/ml fibronectin-coated microwells
WB2 - 10 µg/ml
应用说明 IHC-P: Antigen Retrieval: By Proteinase K.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 272 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 10 mM PBS (pH 7.4) and 1% BSA
稳定剂 1% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 2335 Human FN1

Swiss-port # P02751 Human Fibronectin

基因名称 FN1
基因全名 fibronectin 1
背景介绍 This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
生物功能 Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
翻译后修饰 Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.

检测图片 (1) Click the Picture to Zoom In

  • ARG56127 anti-Fibronectin antibody [FN12-8] IHC-P image

    Immunohistochemistry: Human well-differentiated colon cancer tissue (left) and moderately-differentiated colon cancer (right). Tissues were stained with ARG56127 anti-Fibronectin antibody [FN12-8].