ARG66162

anti-Fibronectin antibody

anti-Fibronectin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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anti-Fibronectin antibody

Mouse Monoclonal antibody recognizes Fibronectin

ARG66162 Arigo biolaboratories
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anti-Fibronectin antibody

概述

产品描述

Mouse Monoclonal antibody recognizes Fibronectin

反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Mouse
克隆 Monoclonal
同位型 IgG
靶点名称 Fibronectin
抗原物种 Human
抗原 Synthetic peptide from Human Fibronectin
偶联标记 Un-conjugated
全名 fibronectin 1
別名 ED-B; CIG; GFND; Cold-insoluble globulin; FNZ; LETS; GFND2; Fibronectin; MSF; FINC; FN

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:300
WB1:1000 - 1:2000
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 272 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14268 Mouse FN1

GeneID: 2335 Human FN1

Swiss-port # P02751 Human Fibronectin

Swiss-port # P11276 Mouse Fibronectin

基因名称 FN1
基因全名 fibronectin 1
背景介绍 This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
生物功能 Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. [UniProt]
翻译后修饰 Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.

检测图片 (9) Click the Picture to Zoom In

  • ARG66162 anti-Fibronectin antibody WB image

    Western blot: 20 µg of Huh7 and Huh7 shHMGB1 cell lysates stained with ARG66162 anti-Fibronectin antibody (1:1000), ARG55914 anti-E-cadherin antibody (1:1000) and ARG65636 anti-HMGB1 antibody (1:2000).

  • ARG66162 anti-Fibronectin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.

  • ARG66162 anti-Fibronectin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG66162 anti-Fibronectin antibody at 1:200 (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. Secondary antibody was diluted at 1:200 (RT, 30min). Negative control: Secondary antibody only.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Human appendix tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

  • ARG66162 anti-Fibronectin antibody IHC image

    Immunohistochemistry: Mouse spleen tissue stained with ARG66162 anti-Fibronectin antibody (red) at 1:200 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A and B.

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anti-Fibronectin antibody

Application:WB

Sample:Huh7 and Huh7 shHMGB1

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC