ARG52280

anti-Fibrillarin antibody [38F3]

anti-Fibrillarin antibody [38F3] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,C. elegans,D. melanogaster ,Plant,S. pombe

Gene Regulation antibody; Nucleolar Marker antibody; DFC Marker antibody; Dense fibrillar component Marker antibody
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概述

产品描述 Mouse Monoclonal antibody [38F3] recognizes Fibrillarin
反应物种 Hu, Ms, Ce, Dm, Plnt, S. pombe
应用 ICC/IF, IHC-Fr, WB
宿主 Mouse
克隆 Monoclonal
克隆号 38F3
同位型 IgG1
靶点名称 Fibrillarin
抗原物种 Yeast
抗原 Yeast nuclear preparations
抗原表位 EYRAWNPFRSKLAAAILGGV
偶联标记 Un-conjugated
別名 rRNA 2'-O-methyltransferase fibrillarin; RNU3IP1; 34 kDa nucleolar scleroderma antigen; FIB; FLRN; EC 2.1.1.-; Histone-glutamine methyltransferase

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100
IHC-Fr1:100
WB1:500 - 1:1000
应用说明 Specific for the ~34kDa Fibrillarin /Nop1p protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Total IgG fraction
缓冲液 Total IgG fraction and 10 mM Sodium azide
抗菌剂 10 mM Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14113 Mouse FBL

GeneID: 2091 Human FBL

Swiss-port # P22087 Human rRNA 2'-O-methyltransferase fibrillarin

Swiss-port # P35550 Mouse rRNA 2'-O-methyltransferase fibrillarin

基因名称 NOP1
背景介绍 Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli . The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin . Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable . This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
产品亮点 Related Antibody Duos and Panels:
ARG30303 Nucleolar Marker Antibody Duo
Related products:
Fibrillarin antibodies; Fibrillarin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related poster download:
Organelle Markers & Loading Control
研究领域 Gene Regulation antibody; Nucleolar Marker antibody; DFC Marker antibody; Dense fibrillar component Marker antibody
预测分子量 34 kDa
翻译后修饰 By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

检测图片 (4) Click the Picture to Zoom In

  • ARG52280 anti-Fibrillarin antibody [38F3] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:500 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG52280 anti-Fibrillarin antibody [38F3] WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG52280 anti-Fibrillarin antibody [38F3] at 1:1000 dilution.

  • ARG52280 anti-Fibrillarin antibody [38F3] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:100 dilution, and costained with ARG52468 anti-Vimentin antibody (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.

    The Fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the Vimentin antibody reveals cytoplasmic intermediate filaments.

  • ARG52280 anti-Fibrillarin antibody [38F3] WB image

    Western blot: HEK293 cytosol, HEK293 nuclear, NIH/3T3 cytosol and NIH/3T3 nuclear fractions stained with ARG52280 anti-Fibrillarin antibody [38F3] (green) at 1:500 dilution.

客户反馈

nuts_pic      Excellent

anti-Fibrillarin antibody [38F3]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

anti-Fibrillarin antibody [38F3]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

anti-Fibrillarin antibody [38F3]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

克隆号文献

A novel mechanism inducing genome instability in Kaposi's sarcoma-associated herpesvirus infected cells.

WB / 

Jackson BR et al.
PLoS Pathog.,  (2014)

publication_link

 

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The transcription factor EGR1 localizes to the nucleolus and is linked to suppression of ribosomal precursor synthesis.

ICC/IF / Human

Ponti D et al.
PLoS One.,  (2014)

publication_link

 

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Def functions as a cell autonomous factor in organogenesis of digestive organs in zebrafish.

IHC-Fr / 

Tao T et al.
PLoS One.,  (2013)

publication_link

 

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Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription.

ICC/IF / Human

Yildirim S et al.
J Cell Sci.,  (2013)

publication_link

 

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Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factors.

ICC/IF / Arabidopsis thaliana

Kammel C et al.
PLoS One.,  (2013)

publication_link

 

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Developmental regulation of nucleolus size during Drosophila eye differentiation.

IHC / Fruit fly (Drosophila melanogaster)

Baker NE et al.
PLoS One.,  (2013)

publication_link

 

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40S ribosome biogenesis co-factors are essential for gametophyte and embryo development.

IHC / Plant

Missbach S et al.
PLoS One.,  (2013)

publication_link

 

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MicroRNA miR-308 regulates dMyc through a negative feedback loop in Drosophila.

WB / Fruit fly (Drosophila melanogaster)

Daneshvar K et al.
Biol Open.,  (2013)

publication_link

 

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