ARG55898

anti-Fatty Acid Synthase antibody

anti-Fatty Acid Synthase antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Hamster

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概述

产品描述 Mouse Monoclonal antibody recognizes Fatty Acid Synthase
反应物种 Hu, Ms, Rat, Hm
预测物种 Mk
应用 ICC/IF, IP, WB
宿主 Mouse
克隆 Monoclonal
同位型 IgG2a
靶点名称 Fatty Acid Synthase
抗原物种 Human
抗原 Purified recombinant Human Fatty Acid Synthase fragments expressed in E.coli.
偶联标记 Un-conjugated
別名 EC 2.3.1.85; EC 1.3.1.39; Fatty acid synthase; EC 2.3.1.41; FAS; EC 4.2.1.59; OA-519; EC 2.3.1.38; EC 2.3.1.39; EC 3.1.2.14; EC 1.1.1.100; SDR27X1

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:400
IPAssay-dependent
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.4), 0.03% Proclin-300 and 50% Glycerol
抗菌剂 0.03% Proclin-300
稳定剂 50% Glycerol
浓度 4.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14104 Mouse FASN

GeneID: 2194 Human FASN

GeneID: 50671 Rat FASN

基因名称 FASN
全名 fatty acid synthase
背景介绍 The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
生物功能 Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. [UniProt]
细胞定位 Cytoplasm; Melanosome
预测分子量 273 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG55898 anti-Fatty Acid Synthase antibody WB image

    Western blot: 30 µg of HeLa and 293T cell lysates stained with ARG55898 anti-Fatty Acid Synthase antibody at 1:1000 dilution.

  • ARG55898 anti-Fatty Acid Synthase antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG55898 anti-Fatty Acid Synthase antibody at 1:400 dilution.

  • ARG55898 anti-Fatty Acid Synthase antibody IP image

    Immunoprecipitation: CHO-K1 cell lysates were immunoprecipitated and stained with ARG55898 anti-Fatty Acid Synthase antibody.

客户反馈

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anti-Fatty Acid Synthase antibody

Application:WB

Sample:3T3

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC