ARG56076
anti-Fascin antibody [SPM133]
anti-Fascin antibody [SPM133] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [SPM133] recognizes Fascin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SPM133 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Fascin |
| 抗原物种 | Human |
| 抗原 | Full length recombinant Human Fascin protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Singed-like protein; SNL; HSN; 55 kDa actin-bundling protein; FAN1; Fascin; p55 |
应用说明
| 应用建议 |
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| 应用说明 | Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FSCN1 |
| 全名 | fascin actin-bundling protein 1 |
| 背景介绍 | This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011] |
| 生物功能 | Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin. |
检测图片 (1) Click the Picture to Zoom In
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ARG56076 anti-Fascin antibody [SPM133] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma stained with ARG56076 anti-Fascin antibody [SPM133].
