ARG64437
anti-FZD7 / Frizzled 7 antibody
anti-FZD7 / Frizzled 7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes FZD7 / Frizzled 7 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FZD7 / Frizzled 7 |
| 抗原物种 | Human |
| 抗原 | C-RFYHRLSHSSKGET |
| 偶联标记 | Un-conjugated |
| 別名 | FzE3; hFz7; Fz-7; Frizzled-7 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 64 kDa |
| 翻译后修饰 | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
检测图片 (2) Click the Picture to Zoom In
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ARG64437 anti-FZD7 / Frizzled 7 antibody WB image
Western blot: HepG2 cell lysate (35 µg protein in RIPA buffer) stained with ARG64437 anti-FZD7 / Frizzled 7 antibody at 0.3 µg/ml dilution.
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ARG64437 anti-FZD7 / Frizzled 7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64437 anti-FZD7 / Frizzled 7 antibody at 5 µg/ml dilution followed by AP-staining.
