ARG41049
anti-FZD6 / Frizzled 6 antibody
anti-FZD6 / Frizzled 6 antibody for Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FZD6 / Frizzled 6 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FZD6 / Frizzled 6 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 19-201 of Human FZD6 (NP_003497.2). |
| 偶联标记 | Un-conjugated |
| 別名 | NDNC10; FZ-6; hFz6; Fz-6; Frizzled-6; HFZ6; FZ6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Mouse kidney | ||||
| 实际分子量 | 110 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FZD6 |
| 全名 | frizzled class receptor 6 |
| 背景介绍 | This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011] |
| 生物功能 | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear (By similarity). [UniProt] |
| 细胞定位 | Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell surface. Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Colocalizes with FZD3 at the apical face of cells (By similarity). [UniProt] |
| 预测分子量 | 79 kDa |
| 翻译后修饰 | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41049 anti-FZD6 / Frizzled 6 antibody WB image
Western blot: 25 µg of Mouse kidney lysate stained with ARG41049 anti-FZD6 / Frizzled 6 antibody at 1:1000 dilution.
