ARG57049
anti-FUBP1 antibody [14F5]
anti-FUBP1 antibody [14F5] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [14F5] recognizes FUBP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 14F5 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | FUBP1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 279-448 of Human FUBP1. |
| 偶联标记 | Un-conjugated |
| 別名 | FBP; Far upstream element-binding protein 1; FUSE-binding protein 1; hDH V; FUBP; DNA helicase V |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96AE4 Human Far upstream element-binding protein 1 |
|---|---|
| 基因名称 | FUBP1 |
| 全名 | far upstream element (FUSE) binding protein 1 |
| 背景介绍 | The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| 生物功能 | Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. [UniProt] |
| 预测分子量 | 68 kDa |
| 翻译后修饰 | Ubiquitinated. This targets the protein for proteasome-mediated degradation. |
检测图片 (2) Click the Picture to Zoom In
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ARG57049 anti-FUBP1 antibody [14F5] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG57049 anti-FUBP1 antibody [14F5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57049 anti-FUBP1 antibody [14F5] WB image
Western blot: 40 µg of 1) HeLa cell lysate, 2) HepG2 cell lysate, 3) Jurkat cell lysate stained with ARG57049 anti-FUBP1 antibody [14F5] at 1:1000.
