ARG64738
anti-FTO antibody
anti-FTO antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes FTO |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog, Rat, Pig |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FTO |
| 抗原物种 | Mouse |
| 抗原 | QQKPDCRPYWEKDD |
| 偶联标记 | Un-conjugated |
| 別名 | EC 1.14.11.-; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; ALKBH9 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9C0B1 Human Alpha-ketoglutarate-dependent dioxygenase FTO |
|---|---|
| 基因名称 | Fto |
| 全名 | fat mass and obesity associated |
| 背景介绍 | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011] |
| 生物功能 | Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. [UniProt] |
| 产品亮点 | Related news: m6A reader YTHDF2 in mRNA decay and aggresome formation; |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 58 kDa |
检测图片 (1) Click the Picture to Zoom In
-
ARG64738 anti-FTO antibody WB image
Western Blot: Human Pancreas lysate (35 µg protein in RIPA buffer) stained with ARG64738 anti-FTO antibody at 0.03 µg/ml dilution.
