ARG42803
anti-FPGS antibody
anti-FPGS antibody for Flow cytometry,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FPGS |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FPGS |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human FPGS. |
| 偶联标记 | Un-conjugated |
| 別名 | Tetrahydrofolylpolyglutamate synthase; EC 6.3.2.17; Folylpolyglutamate synthase, mitochondrial; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | K562 | ||||||
| 实际分子量 | ~ 63 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P48760 Mouse Folylpolyglutamate synthase, mitochondrial Swiss-port # Q05932 Human Folylpolyglutamate synthase, mitochondrial |
|---|---|
| 基因名称 | FPGS |
| 全名 | folylpolyglutamate synthase |
| 背景介绍 | This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014] |
| 生物功能 | Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates. [UniProt] |
| 细胞定位 | Isoform 1: Mitochondrion inner membrane. Mitochondrion matrix. Isoform 2: Cytoplasm. [UniProt] |
| 预测分子量 | 65 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG42803 anti-FPGS antibody WB image
Western blot: K562 cell lysate stained with ARG42803 anti-FPGS antibody at 1:1000 dilution.
