ARG53050

anti-FOXP3 antibody [SP97]

anti-FOXP3 antibody [SP97] for IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody

Overview

Product Description

Rabbit Monoclonal antibody [SP97] recognizes FOXP3

Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SP97
Isotype IgG
Target Name FOXP3
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of human FOXP3 protein.
Conjugation Un-conjugated
Full Name forkhead box P3
Alternate Names XPID; AIID; JM2; IPEX; PIDX; Forkhead box protein P3; Scurfin; DIETER

Application Instructions

Application Suggestion
Application Dilution
IHC-P1:100
Application Note IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Incubation Time: 20 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Tonsil
Calculated MW 50 kDa

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 50943 Human FOXP3

Swiss-port # Q9BZS1 Human Forkhead box protein P3

Gene Symbol FOXP3
Gene Full Name forkhead box P3
Background FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at a low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Cellular Localization Nucleus
Highlight Related products:
FOXP3 antibodies; FOXP3 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody
PTM Polyubiquitinated, leading to its proteasomal degradation in regulatory T-cells (Treg) which is mediated by STUB1 in a HSPA1A/B-dependent manner. Deubiquitinated by USP7 leading to increase in protein stability.
Phosphorylation at Ser-418 regulates its transcriptional repressor activity and consequently, regulatory T-cells (Treg) suppressive function. Dephosphorylated at Ser-418 by protein phosphatase 1 (PP1) in Treg cells derived from patients with rheumatoid arthritis. Phosphorylation by CDK2 negatively regulates its transcriptional activity and protein stability (By similarity).
Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1.
Undergoes proteolytic cleavage in activated regulatory T-cells (Treg), and can be cleaved at either the N- or C-terminal site, or at both sites.

Images (11) Click the Picture to Zoom In

  • ARG53050 anti-FOXP3 antibody [SP97] WB image

    Western Blot: Tonsil stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human B Cell Lymphoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Bladder Transitional Cell Carcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Bladder stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Colon Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Colon stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Lymph Node stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Pancreatic Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Stomach Adenocarcinoma stained with FOXP3 antibody [SP97] (ARG53050)

  • ARG53050 anti-FOXP3 antibody [SP97] IHC-P image

    Immunohistochemistry: Human Thymus stained with FOXP3 antibody [SP97] (ARG53050)