ARG51655

anti-FOXO3A phospho (Ser253) antibody

Datasheet

参考文献2

订购信息

包装

价格 (USD)

100 μl

399

50 μl

279

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes FOXO3A phospho (Ser253)
反应物种	Hu, Ms, Rat
应用	ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	FOXO3A
抗原物种	Human
抗原	Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FOXO3A.
偶联标记	Un-conjugated
別名	FKHRL1; AF6q21; AF6q21 protein; Forkhead box protein O3; Forkhead in rhabdomyosarcoma-like 1; FOXO2; FOXO3A; FKHRL1P2

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:100 - 1:200
IHC-P	1:50 - 1:100
WB	1:500 - 1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液	PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 2309 Human FOXO3 GeneID: 56484 Mouse FOXO3 Swiss-port # O43524 Human Forkhead box protein O3 Swiss-port # Q9WVH4 Mouse Forkhead box protein O3
基因名称	FOXO3
全名	forkhead box O3
背景介绍	Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
生物功能	Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. [UniProt]
研究领域	Gene Regulation antibody
预测分子量	71 kDa
翻译后修饰	In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4/MST1 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. Phosphorylation by AMPK leads to the activation of transcriptional activity without affecting subcellular localization. Phosphorylation by MAPKAPK5 promotes nuclear localization and DNA-binding, leading to induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:10102273, PubMed:11154281, PubMed:16751106, PubMed:17711846, PubMed:18593906, PubMed:21329882). Phosphorylated by CHUK/IKKA and IKBKB/IKKB. TNF-induced inactivation of FOXO3 requires its phosphorylation at Ser-644 by IKBKB/IKKB which promotes FOXO3 retention in the cytoplasm, polyubiquitination and ubiquitin-mediated proteasomal degradation (PubMed:15084260). May be dephosphorylated by calcineurin A on Ser-299 which abolishes FOXO3 transcriptional activity (By similarity). Deacetylation by SIRT1 or SIRT2 stimulates interaction of FOXO3 with SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation (PubMed:21841822). Deacetylation by SIRT2 stimulates FOXO3-mediated transcriptional activity in response to oxidative stress (By similarity). Heavily methylated by SET9 which decreases stability, while moderately increasing transcriptional activity. The main methylation site is Lys-271. Methylation doesn't affect subcellular location. Polyubiquitinated. Ubiquitinated by a SCF complex containing SKP2, leading to proteasomal degradation.

检测图片 (5) Click the Picture to Zoom In

ARG51655 anti-FOXO3A phospho (Ser253) antibody WB image

Western blot: Extracts from NIH/3T3 cells stained with FKHRL1 antibody (Lane 1 and 2) and ARG51655 anti-FOXO3A phospho (Ser253) antibody (Lane 3 and 4).
ARG51655 anti-FOXO3A phospho (Ser253) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51655 anti-FOXO3A phospho (Ser253) antibody.
ARG51655 anti-FOXO3A phospho (Ser253) antibody ICC/IF image

Immunofluorescence: methanol-fixed HeLa cells stained with ARG51655 anti-FOXO3A phospho (Ser253) antibody (Red).
ARG51655 anti-FOXO3A phospho (Ser253) antibody IHC-P image

Immunohistochemistry: Human USMT stained with ARG51655 anti-FOXO3A phospho (Ser253) antibody.

From de Almeida TG et al. Clinics (Sao Paulo). (2024), doi: 10.1016/j.clinsp.2024.100350, Fig. 1. D.
ARG51655 anti-FOXO3A phospho (Ser253) antibody WB image

Western blot: Rat auditory cortex stained with ARG51655 anti-FOXO3A phospho (Ser253) antibody.

From Cai H et al. Int J Mol Med- (2020), doi: 10.3892/ijmm.2020.4453, Fig. 1. D.