ARG54381
anti-FLASH antibody
anti-FLASH antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FLASH |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody recognizes human and mouse FLASH (220kDa). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FLASH |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa 1253-1268 of human FLASH (accession no. AF165161). This sequence differs by one amino acid from that of mouse. |
| 偶联标记 | Un-conjugated |
| 別名 | CASP8-associated protein 2; FLASH; FLICE-associated huge protein; CED-4; RIP25 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP8AP2 |
| 全名 | caspase 8 associated protein 2 |
| 背景介绍 | A mammalian homologue of CED-4 has been identified and designated FLASH (FLICE-associated huge protein). FLASH is involved in Fas-induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH, resulting in activation of caspase-8. FLASH is expressed in multiple tissues and cell types. |
| 生物功能 | Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody |
| 预测分子量 | 223 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54381 anti-FLASH antibody WB image
Western Blot: HeLa stained with ARG54381 anti-FLASH antibody at 0.5 μg/ml dilution.
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ARG54381 anti-FLASH antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG54381 anti-FLASH antibody at 10 μg/ml dilution.
