ARG63657

anti-FHL1 / SLIM1 antibody

anti-FHL1 / SLIM1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody

概述

产品描述 Goat Polyclonal antibody recognizes FHL1 / SLIM1
反应物种 Hu, Ms, Rat
预测物种 Cow, Dog, Pig
应用 FACS, ICC/IF, WB
特异性 This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 FHL1 / SLIM1
抗原物种 Human
抗原 CNKRFVFHNEQVY
偶联标记 Un-conjugated
別名 FHL1A; FHL1B; KYOT; Four and a half LIM domains protein 1; SLIM; FLH1A; XMPMA; FHL-1; SLIM-1; SLIM1; SLIMMER; Skeletal muscle LIM-protein 1

应用说明

应用建议
应用 推荐稀释比
FACS10 µg/ml
ICC/IF10 µg/ml
WB0.05 - 0.3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 14199 Mouse FHL1

GeneID: 2273 Human FHL1

GeneID: 25177 Rat FHL1

背景介绍 This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009]
研究领域 Cell Biology and Cellular Response antibody; Gene Regulation antibody
预测分子量 36 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG63657 anti-FHL1 / SLIM1 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG63657 anti-FHL1 / SLIM1 antibody at 10 µg/ml dilution.

  • ARG63657 anti-FHL1 / SLIM1 antibody WB image

    Western Blot: Human Muscle lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63657 anti-FHL1 / SLIM1 antibody at 0.05 µg/ml dilution.