ARG57146
anti-FH / Fumarase antibody [3F6]
anti-FH / Fumarase antibody [3F6] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [3F6] recognizes FH / Fumarase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3F6 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | FH / Fumarase |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 44-510 of Human FH / Fumarase |
| 偶联标记 | Un-conjugated |
| 別名 | MCUL1; MCL; LRCC; EC 4.2.1.2; Fumarate hydratase, mitochondrial; FMRD; Fumarase; HLRCC |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FH |
| 全名 | fumarate hydratase |
| 背景介绍 | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008] |
| 生物功能 | Also acts as a tumor suppressor. [UniProt] |
| 产品亮点 | Related products: Fumarase antibodies; Fumarase ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| 预测分子量 | 55 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57146 anti-FH / Fumarase antibody [3F6] FACS image
Flow Cytometry: HeLa cell line stained with ARG57146 anti-FH / Fumarase antibody [3F6] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG57146 anti-FH / Fumarase antibody [3F6] WB image
Western blot: 40 µg of 1) HeLa, 2) LNCap, 3) A549, 4) HepG2, 5) 293T, 6) K562, and 7) Jurkat cell lysates stained with ARG57146 anti-FH / Fumarase antibody [3F6] at 1:1000.
