ARG54167

anti-FEN1 antibody

anti-FEN1 antibody for ICC/IF,Western blot and Human,Mouse,Rat,Hamster,Monkey

Gene Regulation antibody

概述

产品描述 Mouse Monoclonal antibody recognizes FEN1
反应物种 Hu, Ms, Rat, Hm, Mk
应用 ICC/IF, WB
宿主 Mouse
克隆 Monoclonal
同位型 IgG1
靶点名称 FEN1
抗原物种 Human
抗原 Purified recombinant human FEN-1 protein fragments expressed in E.coli.
偶联标记 Un-conjugated
別名 hFEN-1; Maturation factor 1; Flap endonuclease 1; DNase IV; EC 3.1.-.-; FEN-1; MF1; Flap structure-specific endonuclease 1; RAD2

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:400
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 45 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 2237 Human FEN1

GeneID: 84490 Rat FEN1

Swiss-port # P39748 Human Flap endonuclease 1

Swiss-port # Q5XIP6 Rat Flap endonuclease 1

基因名称 FEN1
全名 flap structure-specific endonuclease 1
背景介绍 Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair.During DNA replication,cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.It enters the flap from the 5'-end and then tracks to cleave the flap base,leaving a nick for ligation.Also involved in the long patch base excision repair (LP-BER) pathway,by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap.Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions.Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA,and exhibits RNase H activity.Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
生物功能 Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. [UniProt]
细胞定位 Isoform 1: Nucleus > nucleolus. Nucleus > nucleoplasm. Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. Isoform FENMIT: Mitochondrion.
研究领域 Gene Regulation antibody
预测分子量 43 kDa
翻译后修饰 Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

检测图片 (2) Click the Picture to Zoom In

  • ARG54167 anti-FEN1 antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54167 anti-FEN1 antibody at 1:400 dilution.

  • ARG54167 anti-FEN1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG54167 anti-FEN1 antibody at 1:1000 dilution.