ARG41851

anti-FBXO32 antibody [M314]

anti-FBXO32 antibody [M314] for Western blot and Human

概述

产品描述 Rat Monoclonal antibody [M314] recognizes FBXO32
反应物种 Hu
应用 WB
宿主 Rat
克隆 Monoclonal
克隆号 M314
同位型 IgG
靶点名称 FBXO32
抗原物种 Mouse
抗原 Synthetic peptide (coupled to carrier protein) corresponding to aa. 23-35 of Mouse FBXO32.
偶联标记 Un-conjugated
別名 Muscle atrophy F-box protein; Atrogin-1; F-box only protein 32; Fbx32; MAFbx

应用说明

应用建议
应用 推荐稀释比
WB1:500
应用说明 WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
抗菌剂 0.05% Sodium azide
稳定剂 50% Glycerol and 1 mg/ml BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 114907 Human FBXO32

Swiss-port # Q969P5 Human F-box only protein 32

基因名称 FBXO32
全名 F-box protein 32
背景介绍 This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
生物功能 Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. [UniProt]
细胞定位 Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and the nucleus. [UniProt]
预测分子量 42 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG41851 anti-FBXO32 antibody [M314] WB image

    Western blot: Human FBXO32 recombinant protein stained with ARG41851 anti-FBXO32 antibody [M314] at 1:1000, 1:2000 and 1:4000 dilution.