ARG63576
anti-FBL2 / FBXL2 antibody
anti-FBL2 / FBXL2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes FBL2 / FBXL2 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Cow, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FBL2 / FBXL2 |
| 抗原物种 | Human |
| 抗原 | VFSNNDEGLINKK-C |
| 偶联标记 | Un-conjugated |
| 別名 | F-box and leucine-rich repeat protein 2; FBL2; FBL3; F-box/LRR-repeat protein 2; F-box protein FBL2/FBL3 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 47 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG63576 anti-FBL2 / FBXL2 antibody WB image
Western Blot: Human Brain lysates (RIPA buffer, 35 µg total protein per lane) stained with ARG63576 anti-FBL2 / FBXL2 antibody at 0.3 µg/ml dilution.
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ARG63576 anti-FBL2 / FBXL2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cerebral Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63576 anti-FBL2 / FBXL2 antibody at 10 µg/ml dilution followed by AP-staining.
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ARG63576 anti-FBL2 / FBXL2 antibody WB image
Western blot: 35 µg of Human frontal cortex (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63576 anti-FBL2 / FBXL2 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
