ARG52279
anti-FANCI phospho (Ser556) antibody
anti-FANCI phospho (Ser556) antibody for Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FANCI phospho (Ser556) |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Bov, Goat, NHuPrm, Sheep |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FANCI |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Fanconi anemia group I protein; KIAA1794; Protein FACI |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | Specific for the ~150k FANCI protein phosphorylated at Ser556 . Immunolabeling of FANCI is completely eliminated by lambda-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FANCI |
| 全名 | Fanconi anemia, complementation group I |
| 背景介绍 | Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser 556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014). |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 149 kDa |
| 翻译后修饰 | Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding. Phosphorylated in response to DNA damage by ATM and/or ATR. |
检测图片 (1) Click the Picture to Zoom In
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ARG52279 anti-FANCI phospho (Ser556) antibody WB image
Western blot: HeLa cell lysate stained with ARG52279 anti-FANCI phospho (Ser556) antibody showing specific immunolabeling of the ~150kd FANCI protein phosphorylated at Ser556 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the lysate was incubated in λ-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with λ-Ptase.
