ARG45959
anti-FACT / SUPT16H antibody
anti-FACT / SUPT16H antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes FACT / SUPT16H |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | FACT / SUPT16H |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human FACT / SUPT16H. |
| 偶联标记 | Un-conjugated |
| 別名 | SUPT16H; SPT16 Homolog, Facilitates Chromatin Remodeling Subunit; Chromatin-specific transcription elongation factor 140 kDa subunit; FACTP140; FACT complex subunit SPT16; SPT16; FACTp140; CDC68; FACT 140 kDa subunit; Facilitates chromatin transcription complex subunit SPT16; SPT16/CDC68; hSPT16 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 140 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SUPT16H |
| 全名 | SPT16 Homolog, Facilitates Chromatin Remodeling Subunit |
| 背景介绍 | Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009] |
| 生物功能 | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). [UniProt] |
| 细胞定位 | Nucleus; Chromosome. [UniProt] |
| 预测分子量 | 120 kDa |
| 翻译后修饰 | Phosphoprotein; Isopeptide bond; Ubl conjugation; Acetylation; ADP-ribosylation. [UniProt]. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG45959 anti-FACT / SUPT16H antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45959 anti-FACT / SUPT16H antibody at 5 μg/ml dilution.
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ARG45959 anti-FACT / SUPT16H antibody WB image
Western blot: A549 stained with ARG45959 anti-FACT / SUPT16H antibody at 0.5 μg/ml dilution.
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ARG45959 anti-FACT / SUPT16H antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45959 anti-FACT / SUPT16H antibody at 1 µg/10^6 cells dilution.
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ARG45959 anti-FACT / SUPT16H antibody WB image
Western blot: Rat testis stained with ARG45959 anti-FACT / SUPT16H antibody at 0.5 μg/ml dilution.
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ARG45959 anti-FACT / SUPT16H antibody WB image
Western blot: Mouse testis stained with ARG45959 anti-FACT / SUPT16H antibody at 0.5 μg/ml dilution.
